Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: For DKK1-, SPARC-, TIMP2-, COL1, integrin β2, or integrin α3 PLA, purified rabbit monoclonal anti-DKK1 (1:200, Cell Signaling Technology, Cat# 48367S), rabbit polyclonal anti-SPARC (1:200, Cell Signaling Technology, Cat# 5420S), mouse monoclonal anti-TIMP2 (1:200, R&D Systems, Cat# MAB971), mouse monoclonal anti-COL1 (1:200, Cell Signaling Technology, Cat# 66948S), goat polyclonal anti-integrin β1 (1:200, R&D Systems, Cat# AF1778), goat polyclonal anti-integrin β2 (1:200, R&D Systems, Cat# AF1730), and mouse monoclonal anti-integrin α3 (1:200, R&D Systems, Cat# MAB1345) were used.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

Journal: Disease Models & Mechanisms

Article Title: Serum inflammatory cytokines as disease biomarkers in the DE50-MD dog model of Duchenne muscular dystrophy

doi: 10.1242/dmm.049394

Figure Lengend Snippet: Histology of the vastus lateralis muscle from a 6-month-old DE50-MD dog. (A-E) Immunohistochemistry of a section of vastus lateralis muscle showing (A) CCL2 (magenta), (B) CD18 (green) and (C) a merged image of CCL2, CD18 and Hoechst 33342 (blue), with magnified views of the boxed region in C shown for (D) CCL2 and (E) CD18 staining. (F-J) Immunohistochemistry of a serial section showing (F) CCL2 (magenta), (G) MAC387 + regions (green) and (H) a merged image of CCL2, MAC387 and Hoechst 33342 (blue), with magnified views of the boxed region in H shown for (I) CCL2 and (J) CD18 staining. (K) A serial section with H&E staining showing cell infiltrate within and between muscle fibres, fibres with internalised nuclei and hypercontracted fibres (darker magenta). (L) Serial section with acid phosphatase (AP) staining showing positive red staining at areas of cell infiltrate and within some muscle fibres. (M-O) Serial section of immunohistochemistry with no primary antibody as a control with magnified views of the region of inflammation (dashed box) shown for the (N) magenta (CCL2) and (O) green (CD18/MAC387) channels. Scale bar: 100 µm.

Article Snippet: Sections were then incubated with the following combinations of primary antibodies for 1 h at room temperature: goat anti-canine CCL2 IgG (R&D systems, AF1774, 1:20) with rat anti-human perlecan (Millipore, clone A7L6, 1:1000) , mouse anti-canine CD18 (Bio-Rad, monoclonal IgG1, clone CA1.4E9, MA1-82363, 1:100) ( C) or mouse anti-human macrophages (Biorad, monoclonal IgG1, clone MAC387, MCA874GT, 1:100) ( D).

Techniques: Immunohistochemistry, Staining, Control